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Figure 2: Agarose gel electrophoresis of PCR products from paraffin embedded samples. (a) 100 DNA ladder. Neg: Negative control A (No DNA template on the PCR reaction). Column #1. PCR amplification of the paraffin embedded from patient number 4; Column # 2. PCR amplification of the paraffin embedded from patient number 8; Column # 3. PCR amplification of the paraffin embedded from patient number 19; Pos: PCR amplification of the L. major standard strain. (b) 100 DNA ladder. Neg: Negative control A (No DNA template on the PCR reaction). Column # 4. PCR amplification of the paraffin embedded from patient number 14; Column # 5. PCR amplification of the paraffin embedded from patient number 18. Column # 6. PCR amplification of the paraffin embedded from patient number 20. (c) 100 DNA ladder. Neg: Negative control A (No DNA template on the PCR reaction). Column # 7. PCR amplification of the paraffin embedded BAL sample from patient number 13. Column # 8. PCR amplification of the paraffin embedded skin sample from patient number 13

Figure 2: Agarose gel electrophoresis of PCR products from paraffin embedded samples. (a) 100 DNA ladder. Neg: Negative control A (No DNA template on the PCR reaction). Column #1. PCR amplification of the paraffin embedded from patient number 4; Column # 2. PCR amplification of the paraffin embedded from patient number 8; Column # 3. PCR amplification of the paraffin embedded from patient number 19; Pos: PCR amplification of the <i>L. major</i> standard strain. (b) 100 DNA ladder. Neg: Negative control A (No DNA template on the PCR reaction). Column # 4. PCR amplification of the paraffin embedded from patient number 14; Column # 5. PCR amplification of the paraffin embedded from patient number 18. Column # 6. PCR amplification of the paraffin embedded from patient number 20. (c) 100 DNA ladder. Neg: Negative control A (No DNA template on the PCR reaction). Column # 7. PCR amplification of the paraffin embedded BAL sample from patient number 13. Column # 8. PCR amplification of the paraffin embedded skin sample from patient number 13