Indian Journal of Dermatology
DERMATO-MICROBIOLOGY ROUND
Year
: 2007  |  Volume : 52  |  Issue : 1  |  Page : 61--63

Diagnosis of onychomycosis by trypsin treatment method


Immaculata Xess1, Deepti Dubey1, Mathur Purva2 
1 Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
2 Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India

Correspondence Address:
Immaculata Xess
Department of Microbiology, All India Institute of Medical Sciences, New Delhi
India

Background: Fungal infection of the nails is a common, difficult to treat problem, prevalent worldwide. A discrepancy in the microscopic examination and culture findings can create problems in the diagnosis of this common infection. Aim: This study was designed to evaluate a new method for accurate diagnosis of onychomycosis. Materials and Methods: Nail samples from 25 patients of suspected onychomycosis were taken. A portion of the samples was treated with 2% trypsin before culturing and the rest was processed by the standard mycological technique. Results: A higher number of culture positive samples were obtained by the trypsin treatment method as compared to the standard technique. Conclusion: Trypsin treatment prior to culture increases the isolation of fungi from nail samples.


How to cite this article:
Xess I, Dubey D, Purva M. Diagnosis of onychomycosis by trypsin treatment method.Indian J Dermatol 2007;52:61-63


How to cite this URL:
Xess I, Dubey D, Purva M. Diagnosis of onychomycosis by trypsin treatment method. Indian J Dermatol [serial online] 2007 [cited 2020 Dec 2 ];52:61-63
Available from: https://www.e-ijd.org/article.asp?issn=0019-5154;year=2007;volume=52;issue=1;spage=61;epage=63;aulast=Xess;type=0