Indian Journal of Dermatology
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Year : 2010  |  Volume : 55  |  Issue : 4  |  Page : 329-333

Study of reservoir effect of clobetasol propionate cream in an experimental animal model using histamine-induced wheal suppression test

1 Department of Pharmacology, Subharti Medical College, Meerut, India
2 Department of Pharmacology, J.N. Medical College, A.M.U., Aligarh, India
3 Department of Dermatology and Venerology, Institute of Medical Sciences, BHU, Varanasi, India

Correspondence Address:
Afroz Abidi
C/o. S.I.S Abidi, Mass Products India Ltd., Mill Road, Aishbagh, Lucknow 226 004
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0019-5154.74531

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Background: Topical corticosteroids used in various dermatological diseases several times a day led to an increase risk of side effects. By demonstrating a significant reservoir of corticosteroids in the stratum corneum, one can maximize their efficacy and safety as therapeutic agents. Aim: The study was designed to demonstrate a reservoir of topically applied corticosteroid clobetasol propionate cream experimentally in rabbits using histamine-induced wheal suppression test. Materials and Methods: The work was carried out on albino rabbits, as rabbit skin is akin to human skin, using a topical steroid. The topical steroid clobetasol propionate 0.05% cream was applied on the back of rabbit, and after 1-h occlusion histamine-induced wheal suppression test was performed and wheal area measured at 10 min till day 7. Statistical analysis was done by ANOVA followed by "Post Hoc" test. Results: Maximum wheal suppression was seen on day 1 (P < 0.001). Interday comparison of mean wheal size showed no significant difference (P > 0.05) on day 2, 3, and 4 as compared to day 1. Day 5-7 show highly significant difference (P < 0.001) as compared to day 1, thereby suggesting that the reservoir effect of topical clobetasol propionate 0.05% cream persisted till day 4. Conclusions: This work demonstrated that histamine-induced wheal by the topical steroid clobetasol propionate 0.05% cream was suppressed till day 4, indicating that the reservoir of topical corticosteroid persisted till day 4.

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