Indian J Med Microbiol Close
 

Figure 1: HaCaT cells were cultured in different groups: Vehicle control, vascular endothelial growth factor (vascular endothelial growth factor 1 ng/ml, 4 ng/ml, and 8 ng/ml), semaphorin 3A (semaphorin 3A 0.1 μg/ml, 0.5 μg/ml, and 1 μg/ml), vascular endothelial growth factor + semaphorin 3A (vascular endothelial growth factor 4 ng/ml + semaphorin 3A 1 μg/ml), acitretin (acitretin 0.1 μM, 1 μM, and 10 μM), vascular endothelial growth factor + acitretin (vascular endothelial growth factor 4 ng/ml + acitretin 10 μM) for 24 h. Cell viabilities of different groups were measured using the cell counting kit-8 assay. (a) Vascular endothelial growth factor promoted the proliferation of HaCaT cells in a dose-dependent manner and the optical concentration was 4 ng/ml. (b) Semaphorin 3A inhibited the proliferation of HaCaT cells in a dose-dependent manner, the optical concentration was 1 μg/ml. (c) Acitretin inhibited the proliferation of HaCaT cells in a dose-dependent manner and the optical concentration was 10 μM. (d) The optimal effect of each agent was vascular endothelial growth factor at 4 ng/ml, semaphorin 3A at 1 μg/ml and acitretin at 10 μM. Cell proliferation of different groups. *P < 0.05, **P < 0.005 versus the control group. #P < 0.05, ##P < 0.005 versus vascular endothelial growth factor exposed groups

Figure 1: HaCaT cells were cultured in different groups: Vehicle control, vascular endothelial growth factor (vascular endothelial growth factor 1 ng/ml, 4 ng/ml, and 8 ng/ml), semaphorin 3A (semaphorin 3A 0.1 μg/ml, 0.5 μg/ml, and 1 μg/ml), vascular endothelial growth factor + semaphorin 3A (vascular endothelial growth factor 4 ng/ml + semaphorin 3A 1 μg/ml), acitretin (acitretin 0.1 μM, 1 μM, and 10 μM), vascular endothelial growth factor + acitretin (vascular endothelial growth factor 4 ng/ml + acitretin 10 μM) for 24 h. Cell viabilities of different groups were measured using the cell counting kit-8 assay. (a) Vascular endothelial growth factor promoted the proliferation of HaCaT cells in a dose-dependent manner and the optical concentration was 4 ng/ml. (b) Semaphorin 3A inhibited the proliferation of HaCaT cells in a dose-dependent manner, the optical concentration was 1 μg/ml. (c) Acitretin inhibited the proliferation of HaCaT cells in a dose-dependent manner and the optical concentration was 10 μM. (d) The optimal effect of each agent was vascular endothelial growth factor at 4 ng/ml, semaphorin 3A at 1 μg/ml and acitretin at 10 μM. Cell proliferation of different groups. *<i>P</i> < 0.05, **<i>P</i> < 0.005 versus the control group. <sup>#</sup><i>P</i> < 0.05, <sup>##</sup><i>P</i> < 0.005 versus vascular endothelial growth factor exposed groups