Indian Journal of Dermatology
  Publication of IADVL, WB
  Official organ of AADV
 
Users online: 28  
Home  Editorial Board  Current Issue Archives Coming Soon Guidelines Subscriptions  e-Alerts    Login  
    Small font sizeDefault font sizeIncrease font size Print this page Email this page


 
CASE REPORT
Year : 2009  |  Volume : 54  |  Issue : 2  |  Page : 168-172
Clear cell sarcoma: A case mimicking primary cutaneous malignant melanoma


1 Department of Dermatology, Hospital Universitario de Canarias, University of La Laguna, 38320 - La Laguna, Tenerife, Spain
2 Department of Pathology, Hospital Universitario de Canarias, University of La Laguna, 38320 - La Laguna, Tenerife, Spain

Date of Web Publication3-Jul-2009

Correspondence Address:
M Rodriguez-Martin
Dermatology Department, Hospital Universitario de Canarias, Ofra s/n. La Laguna, Santa Cruz de Tenerife, 38320 Canary Islands
Spain
Login to access the Email id


DOI: 10.4103/0019-5154.53193

PMID: 20101313

Get Permissions

   Abstract 

Clear cell sarcoma (CCS) is a recently described variant of sarcoma characterized by prominent clear cells showing features similar to clear cell melanoma. This neoplasm was first described by Dr. Franz M. Erzinger. Primary CCS usually arises in deeper soft tissues, in association with fascia, tendons, or aponeuroses. Characteristic translocation t(12;22) (q13;q12) has been considered pathognomonic for CCS. Prognosis is related to tumor size. An early recognition and initial radical surgery is the key to a favourable outcome. We present a patient with an unusual neoplasm that resembled malignant melanoma.


Keywords: Clear cell sarcoma, genetic studies, malignant melanoma


How to cite this article:
Rodriguez-Martin M, Saez-Rodriguez M, Esquivel B, Gonzalez R S, Cabrera A N, Herrera A M. Clear cell sarcoma: A case mimicking primary cutaneous malignant melanoma. Indian J Dermatol 2009;54:168-72

How to cite this URL:
Rodriguez-Martin M, Saez-Rodriguez M, Esquivel B, Gonzalez R S, Cabrera A N, Herrera A M. Clear cell sarcoma: A case mimicking primary cutaneous malignant melanoma. Indian J Dermatol [serial online] 2009 [cited 2014 Jul 30];54:168-72. Available from: http://www.e-ijd.org/text.asp?2009/54/2/168/53193



   Introduction Top


Clear cell sarcoma (CCS) is a rare neoplasm with a difficult clinical and histological differential diagnosis. The entity of CCS is also known as malignant melanoma of soft parts and it represents about 1% of soft tissue tumors. Because of the presence of melanin, pre-melanosomes, S-100 protein and the tendency for regional nodal metastases, it has been suggested that this entity may be considered as melanoma rather than soft tissue sarcoma. [1] According to some authors, clear cell sarcoma of tendons and aponeuroses should be the proper name for this rare tumor, to prevent confusion with CCS of the kidney and other clear and spindle-cell neoplasms. [2],[3] Clinically, most cases present as a slowly progressive, painless mass on the lower limbs with a predilection for young females. The tumor increases in size followed by metastatic dissemination to lymph nodes and lungs. Malignant melanoma (MM) is the most important differential diagnosis to exclude. However, unusual histologic variants of MM may be problematic to differentiate based on routine histopathologic evaluation. Characteristic translocation t(12;22) (q13;q12) has been considered pathognomonic for CCS. This translocation has been observed in neither cutaneous or uveal MM nor malignant peripheral nerve sheath tumor (MPNSTs+). [3],[4],[5],[6] This genetic traslocation demonstrates that CCS resembles MM but has a different pathogenesis.

An unusual clinical case is presented. We describe a male patient with a cutaneous lesion on the arm, arising in a preexisting pigmented lesion with histological features that resembled melanoma. A rare variant of sarcoma mimicking clear cell melanoma was diagnosed based on the genetic studies. To our knowledge, this is the first reported case of CCS showing intraepidermal involvement.


   Case Report Top


A 53-year-old caucasian male presented to our Department with a painful lesion on the right arm. He related that the pigmented lesion had remained stable for years, with no pain or increase in size. Over the past 4 months, he stated that it began to enlarge and became painful. Medical history was relevant for a pheochromocytoma treated by excisional surgery with adrenalectomy two years ago with no evidence of recurrence. The patient did not have personal or familiar history of cutaneous malignancy.

Physical examination revealed an erythematous, dome- shaped, nodular lesion, 1.3 cm in diameter, firm to palpation and movable with a serohemorragic crust on its surface, located on the right arm [Figure 1]. Clinical examination did not reveal other relevant cutaneous lesions.

Skin biopsy was performed. Histologically, the cutaneous specimen demonstrated epidermal involvement similar to MM. Predominantly, the tumor was composed of oval to polygonal cells with clear cytoplasms and enlarged, irregular, hyperchromatic nuclei with nucleoli. Tumoral cells were arranged in sheets or small nests [Figure 2]a. Junctional activity could be observed in the tumor, with nests of proliferating melanocytic cells showing cytologic atypia in the basal layer. At the lateral margins, atypical melanocytes nested in dermoepidermal junction [Figure 2]b, showing features of a remnant nevus in the specimen. In dermis, cells were disposed in nests separated by fibrous connective tissue, showing an alveolar pattern [Figure 2]c. The depth of invasion was 11mm. Melanin granules were not detected. Mononuclear inflammatory infiltrate, necrotic, and hemorragic areas were also found. Superficial ulceration was observed. Neoplastic cells displayed positivity for HMB-45 and S-100 protein. Immunohistochemistry also showed intraepidermal positivity [Figure 2]d and e. Stains for CEA and cytokeratin were negative. Translocation t(12; 22)(q13; q12) was found in cytogenetic analysis by fluorescence in situ hybridization (FISH). FISH technique was performed using the LSI CHOP (12q13) Dual Color, Break Apart Rearrangement Probe (Abbott Laboratories). Our results showed 20-30% of rearrangement in Chop gene over the analyzed tissue [Figure 3]a and b.

The intraepidermal involvement observed in the histologic study resembled a MM. Nevertheless, the histopathologic, cytogenetic and immunohistochemical profile of this neoplasm was diagnostic of invasive CCS. There was no other identifiable primary cutaneous neoplasm that would give rise to the metastatic deposit. Finally a diagnosis of primary CCS was made based on the strength of the genetic studies.

Axillary lymph node and lung metastases were observed on magnetic resonance imaging. A lung biopsy confirmed the diagnosis of metastatic CCS. Radical surgery, including regional lymph node dissection was performed and adjuvant radiotherapy was instaured.


   Discussion Top


CCS is a recently described variant of sarcoma characterized by prominent clear cell features similar to clear cell melanoma. [7] Primary CCS usually arises in deeper soft tissues, in association with fascia, tendons, or aponeuroses. Histopathologic criteria that support the classification of CCS as a separate entity include the presence of spindle and clear cells, absence of nuclear atypia, and small and inconspicuous nucleoli. Clinically, CCS is most often present in young adults, with a slight female predominance, as a slow-growing but often painful nodule on the extremities. [4],[5],[6] Most cases occur on the lower limbs, with feet predilection. Upper limb is affected in about 25% of cases. [4],[5] Upon incomplete excision, local recurrence and metastasis (lymph nodes, lungs or bones) may occur. [3],[4]

It has been suggested that CCS may be conclusively diagnosed using cytology, immunohistochemistry (HMB- 45+ and S-100+ stains), cytogenetic analysis (demonstrating the specific translocation), and electron microscopy (to supply ultrastructural evidence for the presence of melanosomes). [2],[8],[9],[10] However, CCS shares a similar immunohistochemical profile with MM, with two exceptions: (i) CD68 is more specific for MM and has not been reported positive in CCS, and (ii) CCS displays occasional positivity for chromogranin while MM does not [Table 1]. [3],[11]

Many sarcomas are known to have translocations and gene fusions of great value in specific diagnosis. Characteristic translocation t (12; 22) (q13; q12), has been considered pathognomonic for CCS. [12] This translocation has been identified in 70-90% of CCS cases using cytogenetic studies and reverse-transcriptase polymerase chain reaction. [13] Nevertheless, this cytogenetic rearragement is characteristic but not entirely unique for CCS, because similar fusion genes can also be found in angiomatoid fibrous histiocytoma. [14] In this case, we used FISH technique for cytogenetic studies. It is based on denaturalization and renaturalization of the DNA molecule, by using heat changes and also on the complementarity of base pairs. A single-stranded probe is used to hybridize with a specific DNA sequence of the sample.

To date, this translocation has not been identified in cutaneous MM. However, microsatellite instability (a variation in the lengths of short repeat DNA segments in the genome) and genetic alterations involving chromosomes 1, 5, and 6 have been implicated in MM pathogenesis, but are rare or absent in CCS. [15] Therefore, cytogenetic data could be a clue for establishing the correct diagnosis. [5],[6],[7],[11]

It is important to be aware with the wide range of clinical and pathological differential diagnosis of clear and spindle- cell neoplasms. Most important differential diagnosis is MM, including metastatic MM with unknown primary site or rare variants as clear cell melanoma or balloon cell melanoma. [8],[9],[10]

Follicular dendritic and interdigitating cell tumors, MPNSTs, paraganglioma-like dermal melanocytic tumor, hidradenocarcinoma, perivascular epithelioid cell-omas ( PEC-omas ), clear cell basal cell carcinoma, clear cell syringoma, balloon cell nevus or melanoma, xanthoma, hibernoma, sebaceous neoplasm, atypical fibroxanthoma, or hypernephroma should also be considered. Their common embryologic origin, similar histologic, and immunohistochemical features make differential diagnosis difficult. [3],[4],[5],[6],[16],[17],[18]

Distinction of CCS from metastatic MM is important due to the different treatment and prognosis. [19] Rarity of CCS makes it difficult to draw conclusions regarding prognostic factors. Tumor size ≥5cm, presence of necrosis and perhaps, DNA-index have been found as poor prognosis factors. [1],[20],[21] Regional lymph nodes or lung metastases have been reported in one third of the patients. [3],[4] Five-year survival rates have been estimated to range from 48-67%. [3],[11]

In conclusion, an interesting clinical case of CCS in an adult, arising in the upper limb has been reported. In addition, most important differential diagnoses have been reviewed. The intraepidermal involvement over this tumor (a finding that resembles MM), in a tumor that morphologically resembles MM, in a site that is more typical of melanoma make this final diagnosis difficult. To our knowledge, this is the first reported case of CCS with intraepidermal involvement. In our patient, definitive diagnosis was only possible based on the strength of the genetic studies. Differentiation from the clear or spindle-cell neoplasms, metastatic MM, and unusual MM subtypes is an essential component in patient management. Pathologist and clinicians need to be aware of the aforementioned entities, so that an early diagnosis and treatment may improve the prognosis.

 
   References Top

1.Dewan M, Malatani TS, Ansari MA. Lessons to be learned: A case study approach. Malignant melanoma of soft tissue. J R Soc Health 2005;125:42-6.  Back to cited text no. 1    
2.Crowson A, Magro C, Mihm M. Unusual histologic and clinical variants of melanoma: Implications for therapy. Curr Oncol Rep 2007;9:403-10.  Back to cited text no. 2    
3.Lucas DR, Nascimento AG, Sim FH. Cell sarcoma of soft tissues: Mayo Clinic experience with 35 cases. Am J Surg Pathol 1992;16:1197-204.  Back to cited text no. 3    
4.Enzinger FM. Clear cell sarcoma of tendons and aponeuroses: An analysis of 21 cases. Cancer 1965;18:1163-76.  Back to cited text no. 4    
5.Kawai A, Hosono A, Nakayama R, Matsumine A, Matsumoto S, Ueda T, et al . Clear cell sarcoma of tendons and aponeuroses. A study of 75 patients. Cancer 2007;109:109-16.  Back to cited text no. 5    
6.Hisaoka M, Ishida T, Kuo TT, Matsuyama A, Imamura T, Nishida K, et al . Clear cell sarcoma of soft tissue: A clinicopathologic, immunohistochemical, and molecular analysis of 33 cases. Am J Surg Pathol 2008;5:27-35.  Back to cited text no. 6    
7.Menzel T. Uncommon variants of malignant melanocytic neoplasms. Pathologe 2007;12:  Back to cited text no. 7    
8.Magro CM, Crowson AN, Mihm MC. Unusual variants of malignant melanoma. Mod Pathol 2006;19:S41-70.  Back to cited text no. 8    
9.Zelger BG, Debiec-Rychter M, Sciot R, Zelger B. Cytogenetic comparison between clear cell sarcoma and a case of acral clear cell melanoma. J Dtsch Dermatol Ges 2003;1:363-8.  Back to cited text no. 9    
10.Furuya M, Shimizu M, Nishihara H, Ito T, Sakuragi N, Ishikura H, et al. Clear cell variant of malignant melanoma of the uterine cervix: A case report and review of the literature. Gynecol Oncol 2001;80:409-12.  Back to cited text no. 10    
11.Eckardt JJ, Pritchard DJ, Soule EH. Clear cell sarcoma: A clinicopathologic study of 27 cases. Cancer 1983;52:1482-8.  Back to cited text no. 11    
12.Panagopoulos I, Mertens F, Isaksson M, Mandahl N. Absence of mutations of the BRAF gene in malignant melanoma of soft parts (clear cell sarcoma of tendons and aponeuroses). Cancer Genet Cytogenet 2005;156:74-6.  Back to cited text no. 12    
13.Patel RM, Downs-Kelly E, Weiss SW, Folpe AL, Tubbs RR, Tuthill RJ, et al. Dual-color, break apart fluorescence in situ hybridization for EWS gene rearrangement distinguishes clear cell sarcoma of soft tissue from malignant melanoma. Mod Pathol 2005;18:1585-90.  Back to cited text no. 13    
14.Meis Kindblom JM. Clear cell sarcoma of tendons and aponeuroses: A historical perspective and tribute to the man behind the entity. Adv Anat Pathol 2006;13:286-92.  Back to cited text no. 14    
15.Garcia JJ, Kramer MJ, OŽDonell RJ, Horvai AE. Mismatch repair proteνna and microsatellite instability: A comparison of clear cell sarcoma of soft parts and metastαtico melanoma. Mod Pathol 2006;19:950-7.  Back to cited text no. 15    
16.Deyrup AT, Althof P, Zhou M, Morgan M, Solomon AR, Bridge JA, et al . Paraganglioma-like dermal melanocytic tumor: A unique entity distinct from cellular blue nevus, clear cell sarcoma and cutaneous melanoma. Am J Surg Pathol 2004;28:1579-86.  Back to cited text no. 16    
17.Schmuth M, Spφtl L, Zelger B, Weinlich G, Zelger B. Clear cells in acral melanoma. Eur J Dermatol 2001;11:21-4.  Back to cited text no. 17    
18.Kao GF, Helwig EB, Graham JH. Ballon cell malignant melanoma of the skin. A clinicopathological study of 34 cases with histocehemichal, immunohistochemichal and ultrastructural observations. Cancer 1992;69:2942-52.  Back to cited text no. 18    
19.Marquιs B, Terrier P, Voigt JJ, Mihura J, Coindre JM. Clear cell soft tissue sarcoma: Clinical, histopathological and prognostic study of 36 cases. Ann Pathol 2000;20:298-303.  Back to cited text no. 19    
20.Graadt van Roggen JF, Mooi WJ, Hogendoorn PC. Clear cell sarcoma of tendons and aponeuroses (malignant melanoma of soft parts) and cutaneous melanoma: Exploring the histogenic relationship between these two clinicopathological entities. J Pathol 1998;186:3-7.  Back to cited text no. 20    
21.Deenik W, Mooi W, Rutgers E, Johannes P, Hart A, Kroon B. Clear cell sarcoma (malignant melanoma) of soft parts: A clinicopathological study of 30 cases. Cancer 1999;86:969-75.  Back to cited text no. 21    


    Figures

  [Figure 1], [Figure 2], [Figure 3]
 
 
    Tables

  [Table 1]

This article has been cited by
1 Superficial paramucosal clear cell sarcoma of the soft parts resembling melanoma in a 13-year-old boy
Sidiropoulos, M. and Busam, K. and Guitart, J. and Laskin, W.B. and Wagner, A.M. and Gerami, P.
Journal of Cutaneous Pathology. 2013; 40(2): 265-268
[Pubmed]
2 Superficial paramucosal clear cell sarcoma of the soft parts resembling melanoma in a 13-year-old boy
Michael Sidiropoulos,Klaus Busam,Joan Guitart,William B. Laskin,Annette M. Wagner,Pedram Gerami
Journal of Cutaneous Pathology. 2013; 40(2): 265
[Pubmed]
3 Two cases of clear cell sarcoma with different clinical and genetic features: cutaneous type withBRAFmutation and subcutaneous type withKITmutation
B.M. Park,S.A. Jin,Y.D. Choi,S.H. Shin,S.T. Jung,J.B. Lee,S.C. Lee,S.J. Yun
British Journal of Dermatology. 2013; 169(6): 1346
[Pubmed]
4 Cutaneous Clear Cell Sarcoma
Giovanni Falconieri,Carlos E. Bacchi,Bostjan Luzar
The American Journal of Dermatopathology. 2012; 34(6): 619
[Pubmed]
5 A case of cutaneous clear cell sarcoma determined by clinicopathological and cytogenetic analysis
Yung-Ting Chuang, Tze-Yi Lin, Chih-Jung Hsu
Dermatologica Sinica. 2012;
[VIEW]
6 A case of malignant melanoma in the temporal muscle
Miyano, K. and Yasuhara, K. and Monobe, H. and Tojima, H.
Practica Oto-Rhino-Laryngologica. 2012; 105(9): 863-868
[Pubmed]
7 Cutaneous clear cell sarcoma: Report of three cases of a potentially underestimated mimicker of spindle cell melanoma
Falconieri, G. and Bacchi, C.E. and Luzar, B.
American Journal of Dermatopathology. 2012; 34(6): 619-625
[Pubmed]



 

Top
Print this article  Email this article
 
  Search
 
   Next article
   Previous article 
   Table of Contents
  
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Article in PDF (734 KB)
    Citation Manager
    Access Statistics
    Reader Comments
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
    Introduction
    Case Report
    Discussion
    References
    Article Figures
    Article Tables

 Article Access Statistics
    Viewed3340    
    Printed128    
    Emailed2    
    PDF Downloaded156    
    Comments [Add]    
    Cited by others 7    

Recommend this journal

Site supported by Galderma