Indian Journal of Dermatology
  Publication of IADVL, WB
  Official organ of AADV
Indexed with Science Citation Index (E) , Web of Science and PubMed
 
Users online: 2073  
Home About  Editorial Board  Current Issue Archives Online Early Coming Soon Guidelines Subscriptions  e-Alerts    Login  
    Small font sizeDefault font sizeIncrease font size Print this page Email this page


 
ORIGINAL ARTICLE
Year : 2006  |  Volume : 51  |  Issue : 4  |  Page : 265-268
Nail unit in collagen vascular diseases: A clinical, histopathological and direct immunofluorescence study


Department of Dermatology, Kasturba Medical College, Manipal - 576104, India

Correspondence Address:
Raghavendra Rao
Department of Dermatology, Kasturba Medical College, Manipal - 576104
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0019-5154.30291

Rights and Permissions

   Abstract 

Background: Abnormalities of the nail unit are common in patients with connective tissue diseases. Clinical examination of the nail unit, coupled with biopsy of proximal nail fold offers an additional advantage in the diagnosis. Purpose: Our aim was to record clinical changes of the nail unit in connective tissue diseases and to study the histopathological (both H and E and periodic acid Schiff and direct immunofluorescence (DIF) findings of nail-fold biopsy. Materials and Methods: Thirty-eight confirmed cases connective tissue diseases attending skin OPD were enrolled in the study. After detailed clinical examination of the nail unit, a crescentric biopsy was taken from the proximal nail fold (PNF). Histopathological and DIF studies were was carried out. Findings: Nail changes could be demonstrated in 65% connective tissue diseases. Specific histopathological (H and E) and immunofluorescence findings were also encountered in many patients. Conclusion: Clinical examination of the nail unit offers additional clue in the diagnosis of connective tissue diseases. Though DIF of PNF biopsy is useful in the diagnosis, it is not an ideal site for H and E study, as the yield is very low. Limitations: Lack of adequate comparison group and non-utilization of capillary microscopy for the detection of nail fold capillary abnormalities.


Keywords: Direct immunofluorescence, nail-fold telangiectasia, periodic acid Schiff positive cuticular deposits, proximal nail fold biopsy


How to cite this article:
Nabil P A, Rao R, Shenoi SD, Balachandran C. Nail unit in collagen vascular diseases: A clinical, histopathological and direct immunofluorescence study. Indian J Dermatol 2006;51:265-8

How to cite this URL:
Nabil P A, Rao R, Shenoi SD, Balachandran C. Nail unit in collagen vascular diseases: A clinical, histopathological and direct immunofluorescence study. Indian J Dermatol [serial online] 2006 [cited 2019 Jun 26];51:265-8. Available from: http://www.e-ijd.org/text.asp?2006/51/4/265/30291



   Introduction Top


Examination of the nail apparatus in patients with connective tissue diseases may provide important information as they may have broad spectrum of nail changes.[1] Because of the translucency of the nail plate, the proximity of its vasculature and the fact that the nail complex frequently manifests changes associated with connective tissue diseases, the nail should be scrutinized routinely when examining the patient. The common changes include periungual erythema, nail fold telangiectasia (NFT), ragged cuticle, finger tip scarring etc. Thus, nail findings give an important clue to the diagnosis.


   Materials and Methods Top


Thirty-eight consecutive patients of connective tissue diseases (diagnosis confirmed by biopsy and serology) attending skin OPD were chosen for the study. After a detailed clinical examination of the nail unit, a crescentric biopsy was taken from the proximal nail fold (PNF); either from the involved skin or apparently normal skin.

Biopsy technique

Informed consent was taken and the biopsy site was anaesthetized using 2% lignocaine. A spatula was inserted under the PNF and the tissue loosened from the underlying nail plate over approximately 2-3 mm from the free edge of the nail fold on the midline decreasing gradually towards the lateral nail folds. A crescent - shaped section of the proximal nail fold was then cut. It was divided in to two halves, one stained for histopathology (H and E and PAS) and the other for direct immunofluorescence (DIF).


   Results Top


Out of 38 patients, four were males and 34 were females. The diagnostic distributions of the patients are shown in [Table - 1]. Nail changes were observed in 25 (65.8%) patients [Table - 2]. The most common nail changes observed were the presence of NFT and longitudinal ridging (28.9% each). Other changes observed include, nail-fold hyperpigmentation (21.1%), nail-fold erythema (18.4%), ragged cuticle and discoloration (15.8%), fingertip scar and chronic paronychia (7.9%), nail fold depigmentation (5.3%), pitting, cuticular hypertrophy and digital gangrene (2.6% each) [Table - 3].

Scleroderma

Out of seven female patients with scleroderma, four had diffuse cutaneous type while the rest had limited cutaneous type. Nail changes were observed in five patients (71.4%); four with diffuse cutaneous type and one with limited cutaneaous type. The most common change noted was NFT (four patients; 57.1%). Other changes observed were ragged cuticle (three patients; 42.86%), fingertip scars (three patients; 42.86%), longitudinal ridging (two patients; 28.6%).

Mixed connective tissue disease (MCTD)

Nail changes were observed in six patients (85.7%). NFT was the commonest finding (four patients; 57%), followed by longitudinal ridging, cuticular hypertrophy, discoloration of nail plate, nail fold depigmentation and hyperpigmentation. Digital gangrene was observed in one.

Systemic lupus erythematosus (SLE)

Out of 11 (61.1%) patients with nail changes, six had nail-fold erythema (54.5%) [Figure - 1]. Other changes noted were longitudinal ridging, ragged cuticle, NFT, pitting, discoloration, chronic paronychia, nail-fold hyper and depigmentation.

Other conditions

NFT was seen in one patient of DM. One patient with DLE had chronic paronychia with discoloration of the nail, while nail changes in the form of longitudinal ridging, thinning and nail-fold hyperpigmentation was seen in one patient of rheumatoid arthritis.

Raynaud's phenomenon

Raynaud's phenomenon was seen in eight patients (21.05%). This included three cases each of SLE and MCTD and two cases of scleroderma. All of them showed nail changes.

Histopathology

Specific H and E changes of connective tissue diseases were seen in seven patients (18.4%). Homogenization of the collagen was seen in four patients with scleroderma [Figure - 2] while basal cell degeneration was seen in three samples of which two had SLE and one MCTD.

PAS positive cuticular deposits [Figure - 3] were present in 10 (26.3%) nail-fold biopsy samples, out of which 8 had nail changes. They were more common and were more extensive in scleroderma (three patients; 41.9%); they were also seen in SLE (3 patients; 41.9%), MCTD and DLE (two patients each; 33.3%).

DIF

DIF of nail-fold was positive in 27 (71.05%) samples. Lupus band (with immunoglobulin, C3 and fibrinogen) at dermo-epidermal junction was positive in 22 (57.8%) samples [Figure - 4]. This included 13 (72.2%) samples of SLE, five samples of scleroderma (71.4%), three samples of MCTD (42.9%) and one sample of DLE (33.3%). This was more common with IgM than with other FITC conjugates. Other DIF changes noted were blood vessel deposits (C3, fibrinogen) in 9 (23.7%) patients (MCTD - 3, SLE - 2, DM-2, RA and DLE - one each), epidermal nuclear stain in three cases, (two cases of MCTD and one case of SSc) and IgM colloid bodies (DLE, SLE - one case each).


   Discussion Top


Nails may be affected either directly or indirectly in various connective tissue diseases. The vast majority of these onychopathies represent non-specific reaction pattern. But a few changes are more specific and help to establish the diagnosis. Hence a thorough examination of the nail unit is mandatory in all patients with connective tissue diseases.

Commonest nail change observed in our patients with SLE was nail-fold erythema. This is in contrast to the previous study by Urowitz et al . who reported onycholysis to be the commonest change.[2] They also found that the incidence of Raynaud's phenomenon and mucous membrane ulcerations were common in SLE patients who had nail changes. Other reported signs like red lunula,[3] bluish-black discoloration of the nail plate[4] were not seen in our patients.

NFT was seen most frequently in scleroderma and MCTD patients in the study. Previous studies have found an association between the degree of nail fold capillary abnormality (as detected by capillary microscopy) and internal organ involvement.[5],[6],[7] Systemic organ involvement in the form of interstitial lung diseases was noted in two of our patients with scleroderma who had NFT. Maricq et al . have observed that long term follow-up of patients with Raynaud's disease is necessary when they have associated NFT as they are likely to develop systemic manifestations later in the course of the disease.[5] Thus examination of the proximal nail-fold gives useful diagnostic and prognostic information in patients with connective tissue diseases.

Out of two patients with dermatomyositis, only one had NFT. Thickening and hyperkeratosis of the cuticle are the other common nail changes that were not seen in our patients.[8] One patient of rheumatoid arthritis in our study had nail changes in the form of thinning, hyperpigmentation and longitudinal ridging. Other characteristic nail changes associated with rheumatoid arthritis like red lunula, splinter hemorrhages, periungual vascular lesions were not seen in our patients.[9],[10]

There is paucity of reports on nail-fold biopsies in the literature. This may be partly due the concern that biopsies of this site could result in disfigurement of the nails. Also, there may be concern regarding the poor healing of the biopsy site in scleroderma patients. However it is a well-established fact that compared to 'spontaneous' ulceration, biopsy site heal surprisingly well. Our study further supported this fact as all our patients had normal healing. Specific histopathological (H and E) changes were observed in 18% while eosinophilic PAS positive cuticular deposits were noted in 26% of the samples. This cuticular deposit occurs due to increased vascular permeability resulting in the extravascular deposition of serum proteins. Scher et al. have observed that the severity of cuticular deposits correlates with the degree of in vivo capillary abnormalities.[11] DIF of the nail-fold showed typical lupus band in 57.8% of our patients. Chen et al. have described a specific granular epidermal intercellular immunoglobulin deposition and a unique cytoplasmic staining pattern in dermatomyositis.[12] We did not encounter such findings.


   Conclusion Top


Nail-fold biopsy is a simple procedure that can be performed with minimal or no disfigurement of the finger. Moreover, equal halves of the same biopsy specimen can be used for both DIF and histopathological (H and E and PAS) evaluation.

 
   References Top

1.De Berker DA, Baran R, Dawber RP. The nail in systemic diseases and drug induced changes. In : Handbook of Diseases of the nail and their management. Blackwell Science: Oxford; 1995. p. 85-113.  Back to cited text no. 1      
2.Urowitz MB, Gladman DD, Chalmers A, Ogryzlo MA. Nail lesions in systemic lupus erythematosus. J Rheumatol 1978;5:441-7.  Back to cited text no. 2  [PUBMED]    
3.Wollina U, Barta U, Uhlemann C, Oelzner P. Lupus erythematous associated red lunula. J Am Acad Dermatol 1999;41:419-21.  Back to cited text no. 3  [PUBMED]  [FULLTEXT]  
4.Vaughn RY, Bailey JP Jr, Field RS, Loebl DH, Mealing HG Jr, Jerath RS, et al . Diffuse nail dyschromia in black patients with systemic lupus erythematosus. J Rheumatol 1990;17:640-3.  Back to cited text no. 4      
5.Maricq HR, Spencer-Green G, LeRoy EC. Skin capillary abnormalities as indicators of organ involvement in scleroderma (systemic sclerosis), Raynaud's syndrome and dermatomyositis. Am J Med 1976;61:862-70.  Back to cited text no. 5  [PUBMED]    
6.Schimidt KU, Mensing H. Are nail fold capillary changes indicators of organ involvement in progressive systemic sclerosis? Dermatologica 1988;176:18-21.  Back to cited text no. 6      
7.Groen H, Wichers G, ter Borg EJ, van der Mark TW, Wouda AA, Kallenberg CG. Pulmonary diffusing capacity disturbances are related to nail fold capillary changes in patients with Raynaud's phenomenon with or without an underlying connective tissue disease. Am J Med 1990;89:34-41.  Back to cited text no. 7      
8.Samitz MH. Cuticular changes in dermatomyositis. Arch Dermatol 1974;110:866-7.  Back to cited text no. 8      
9.Jorizzo JL, Gonzalez EB, Daniel HC. Red lunula in a patient with rheumatoid arthritis. J Am Acad Dermatol 1983;8:711-4.  Back to cited text no. 9      
10.Hamilton EB. Nail studies in rheumatoid arthritis. Ann Rheum Disease 1960;19:167-73.  Back to cited text no. 10      
11.Scher RK, Tom DW, Lally EV, Bogaars HA. The clinical significance of periodic acid Schiff - positive deposits in cuticle - proximal nail fold biopsy specimen. Arch Dermatol 1985;121:1406-9.  Back to cited text no. 11      
12.Chen Z, Maize JC, Silver RM, Dobson RL, Maricq HR, Ainsworth SK. Direct and indirect immunofluorescent findings in dermatomyositis. J Cutan Pathol 1985;12:18-27.  Back to cited text no. 12      


    Figures

  [Figure - 1], [Figure - 2], [Figure - 3], [Figure - 4]
 
 
    Tables

  [Table - 1], [Table - 2], [Table - 3]



 

Top
Print this article  Email this article
 
  Search
 
   Next article
   Previous article 
   Table of Contents
  
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Article in PDF (625 KB)
    Citation Manager
    Access Statistics
    Reader Comments
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
    Introduction
    Materials and Me...
    Results
    Discussion
    Conclusion
    References
    Article Figures
    Article Tables

 Article Access Statistics
    Viewed9852    
    Printed136    
    Emailed2    
    PDF Downloaded222    
    Comments [Add]    

Recommend this journal